Development Of Validated Stability Indicating Method By Rp-Hplc For Simultaneous Estimation Of Meropenem And Vaborbactam In Bulk And Pharmaceutical Formulation

Objective: To develop a simple, rapid, precise and reproducible liquid chromatographic method for the estimation meropenem (MEP) and vaborbactam (VAB) in bulk and pharmaceutical formulation and study of the stability of the drugs in different stressed conditions. Methods: The chromatographic separation was achieved on a Kromasil C18 column (250 × 4.6 mm) using a mobile phase composition of acetonitrile and 10 mmol phosphate buffer (pH 3.50) in a ratio 30:70 v/v, pumped at a flow rate of 1.0 ml/min with UV detection set at 260 nm. Results: Symmetrical and sharp peaks of MEP and VAB were obtained at retention times of 2.29 and 3.10 min, respectively. The chromatographic method was validated for linearity, limits of detection and quantitation, precision, accuracy, system suitability and robustness. Calibration curves were obtained in the concentration ranges of 25–150 μg/ml for MEP and VAB. Stability tests done through the exposure of the analytes solution for different stress conditions and the obtained results indicate no interference of degradants with HPLC method. Conclusion: The proposed method has been found to be selective, precise, linear, accurate, and sensitive. The method can be successfully applied to the assay determination of bulk drugs and combined dosage forms for routine analysis.

Knowledge of Basic Life Support among the Nursing Staff and Students of KIMSDU.

Background: Nursing staff plays important role in the health care system; one of the key skills that students should develop during their training is to be prepared for emergency life saving measures like cardiopulmonary resuscitation (CPR) anytime, anywhere. At least the doctors, nursing and paramedical staff are expected to know about it, as they frequently face life threatening situations. Objectives: a) To evaluate the CPR awareness among nursing staff and students. b) To screen the knowledge regarding accurate, effective CPR procedural techniques and various barriers of CPR failure in clinical practice from a student perspective. Methods: A cross-sectional Observational study conducted among nursing staff and students of the Krishna institute of nursing science, Karad. Maharashtra. Results: No one has complete knowledge of BLS. Only 3% of total students have 80% knowledge about BLS.81% of the students has less than 50% of knowledge.Conclusion:It can be concluded that not only the nursing staff and students be trained in Basic Life Support, but also it has to be reinforced from time to time, since the skills of CPR are difficult to teach and once taught difficult to retain. As these are the persons, who are near the patient for 24 hrs easily approachable with in no time till the resident or the CMO attends the patient in the hospital. Also, this should be incorporated into the academic curriculum, as it will become a compulsory topic to be dealt with specially for the nursing students.

Pseudomyxoma peritonei presenting as sub-acute large bowel obstruction.

A case of pseudomyxoma peritonei, a rare mucin-secreting tumour in the peritoneum, in a 30-year-old male and presenting as sub-acute large bowel obstruction, is reported here with review of the literature.

Primary adenocarcinoma of the vermiform appendix: a case report.

Primary adenocarcinoma of the appendix is rare, and since Berger first recognised the neoplasm in 1882, fewer than 250 cases have been recorded. Adenocarcinoma of the appendix is never suspected pre-operatively, being usually first discovered by histological examination. Ileocaecal resection during the first operation and right hemicolectomy for a carcinoma diagnosed after appendicectomy remain the main stay of treatment.

Simple closure of perforated duodenal ulcer.

A study was undertaken to evaluate the efficacy of simple closure followed by drug therapy in cases of perforated duodenal ulcer and to identify the risk factors in relation to the mortality. The male to female ratio was 5:1. Of the patients 59.2% were up to 50 years of age, while 40.8% were above the age of 50 years. Of the patients 47% were admitted 24 hours after the onset of peritonitis. All these patients were surgically treated with simple closure of the perforation with omental patch. Nine patients expired leading to 7.5% mortality. The risk factors identified for mortality were age 60 years and above, presence of shock on admission and delayed presentation. The mortality is directly related to the number of risk factors present in a given patient. At the time of discharge the patients were advised to take orally famotidine 40 mg at bed time for a period of 8 weeks. Eighty-one patients could be followed up and Visick grading was done. Sixty-two patients were in grade I, 11 in grade II, 3 in grade III and 5 in grade IV. The results indicate that simple closure followed by drug therapy is acceptable treatment for perforated duodenal ulcer.